| OBJECTIVE |
Experienced Interdisciplinary Research Scientist
During my career as a scientist in biotech, pharmaceutical and academic research, I discovered a strong interest and strength in developing and applying new technologies to advance the scientific discovery process. Trained as a biophysicist, with experience in molecular and cellular biology, genetics, biochemistry and pharmacology, I have a well-rounded knowledge base and skill set. I see the realization of my professional potential in an interdisciplinary environment with a strong implementation of innovative research and state-of-the-art technologies. My dedication to quality, accuracy and efficient work organization stem from a desire to deliver excellence throughout my daily efforts as part and leader of a strong team. I am adept at problem solving, and look forward to the unique challenges and opportunities inherent in a new position.
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| EDUCATION AND ACADEMIC DEGREES |
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| PhD in Biophysics (summa cum laude), HUMBOLDT UNIVERSITY, Berlin, Germany |
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1992 - 1998
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| Diploma degree in Biophysics (M.S. equivalent), HUMBOLDT UNIVERSITY |
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1987 - 1992
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| PROFESSIONAL EXPERIENCE AND ACHIEVEMENTS |
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Director of Biotechnology, GENOMAS, INC., Hartford, CT
Gualberto Ruano, MD, PhD, CEO and President
- Proposal and grant development: Authored and co-authored 8 small business grants (SBIR) and several research foundation grant. Current approved funding $170,000, 02/2005.
- Designed and managed genomic projects: Planned and executed physio- and pharmacogenomic clinical research studies in the areas of metabolic syndrome, diabetes, diet and exercise physiology, taste, neuropsychiatry, drug-induced metabolic syndrome, statin-induced myalgias.
- Designed and set up genetics research and clinical laboratory: Made key technology and equipment decisions, established research protocols and procedures, adapted them to specific projects and tested their applicability.
- Implemented state-of-the-art technologies: Set-up high-throughput genotyping on Illumina BeadStation and clinical genotyping and proteomics on Luminex platform. Customized workflow and sample processing. Designed genotyping arrays for up to 1500 SNPs.
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2004 - 2005
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Visiting Scientist, HARTFORD HOSPITAL, Preventive Cardiology, Hartford, CT
Paul D. Thompson, MD, Director of Preventive Cardiology, Professor of Medicine at the University of Connecticut School of Medicine
- Liaison between Genomas and Hartford Hospital: Supported research and clinical projects in the areas of cardiovascular genetics, cell biology, molecular biology and physiology.
- Analyzed and evaluated medical records of over 1,500 patients.
- Developed searchable database with patient entries (e.g., demographics, clinical history, medication).
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2004 - 2005
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Post-Doctoral Fellow, YALE MEDICAL SCHOOL, Pharmacology, New Haven, CT
William C. Sessa, PhD, Professor of Pharmacology, Director Vascular Cell Signaling and Therapeutics Program
- Established expertise in cardiovascular biology, including angiogenesis and apoptosis: Designed and evaluated in vitro and ex vivo models to study physiological and disease related processes.
- Designed and evaluated model systems, in vitro and ex vivo, to study physiological and disease related processes of the endothelium.
- Proved efficacy with large variety of laboratory methods and procedures: Proved efficacy in operating in vitro laboratory covering cell biology, biochemistry, gene transfer, biophysics, molecular biology.
- Established and developed new methods and procedures to improve technology portfolio of laboratory (e.g., RNA interference, protein identification and quantification with Li-Cor Odyssey Infrared Imaging System).
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1999 - 2003
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Visiting Scientist, BERLEX BIOSCIENCES, Cardiovascular Research, Richmond, CA
Gabor Rubanyi, MD, PhD, Vice President
- Improved technologies to quantify nitric oxide and oxygen free radical release from cultured cells.
- Studied pathology of endothelial dysfunction and evaluated pharmacological intervention.
- Supported drug discovery projects: Participated in decision making process, studied pathological implications of endothelial dysfunction and evaluated drugs for intervention.
- Transferred assays to high-throughput screening format.
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1995 - 1999
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Graduate Student, RESEARCH INSTITUTE OF MOLECULAR PHARMACOLOGY, Department of Cellular Biochemistry, Berlin, Germany
Ingolf E. Blasig, PhD, Laboratory Head
- Performed independent research for PhD thesis: "Investigations on the regulation of endothelial nitric oxide formation with emphasis on the interaction of nitric oxide and superoxide".
- Established technologies for sensitive and specific detection and quantification of nitric oxide.
- Explored novel pharmacological interventions: Characterized pharmacological properties of substances, documented potential use of spin trapping agents as scavengers of free radicals.
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1992 - 1994
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Undergraduate Student, HUMBOLDT UNIVERSITY, Department of Biology, Division of Biophysics, Berlin, Germany
Ingolf Bernhardt, PhD, Laboratory Head
- Performed independent research for Diploma thesis: "Influence of photosensitizers on the potassium influx in human erythrocytes".
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1987 - 1992
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| SKILLS PROFILE |
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Management and Organization
• Grant writing (business / research grants)
• Planning of clinical studies
• Coordination of research collaborations
• Support of business development
• Planning, establishment and supervision of research and clinical laboratory operations
Biochemistry and Molecular Biology
• Protein analysis (i.e., Electrophoresis,
Western Blot, Dot-Blot, ELISA)
• Immuno- and co-immunoprecipitations
• Characterization of protein phosphorylation and activity (i.e., in vitro kinase activity assays)
• Investigation of subcellular organelles and structures (i.e., lipid rafts, caveolae)
• Yeast-two-hybrid interaction screening
• Lipid and protein separation and analysis (HPLC, FPLC)
• Molecular Biology techniques
Cell biology
• RNA interference and DNA anti-sense
• Extensive cell and tissues culture expertise
• Transient and stable transfections
• Adeno- and retroviral gene delivery
• Evaluation of signal transduction pathways
• Immunofluorescence microscopy, flow cytometry
• Assays for apoptosis, cytotoxicity, cell adhesion, migration, proliferation, Hypoxia/reoxygenation
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Genomics
• High-content, high-throughput genotyping (Illumina BeadArray technology)
• Clinical genotyping (Luminex xMAP)
• DNA preparation, amplification and QC
• Selection and validation of SNPs using bioinformatics databases and programs
Pharmacology and Biophysics
• Organ chamber studies (aortic ring)
• Isolated perfused heart
• Receptor evaluation by microphysiometery
• Assay adaptation for High-Throughput Screening
• NO chemiluminescence
• Spin trapping and EPR spectroscopy
• Chemiluminescent and spectrophotometric techniques (i.e., detection of free radicals)
• Evaluation of spin traps for in vitro studies
• Assays to characterize antioxidative capacity of pharmacological agents
• Radioactive tracers experiments
Computer Skills
• Data acquisition systems and software
• Database handling, Statistical data analysis
• High proficiency in Microsoft Office, scientific graphic, and desktop publishing software
Languages
• German and English (fluent)
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